Having a railway museum located in Odense was conceived by the then director general of the State Railways P.E.N. Skov. Odense was chosen because of its central location and its facilities with a roundhouse just next to Odense's Central Station. The Museum has become a tourist attraction and has an increasing number of visitors because of the many alternating exhibitions. The DSB Railway Museum in Odense opened its doors to the public on april 17th 1975.

The exhibitions of the museum show the history of Denmark's railways and ferries from 1844 to the present. An area of about 5000 m2  contains royal saloon cars, cars and locomotives - both steam and diesel. There is also a Wagons-Lits dining car, platform scenario from approx. 1890, driver's seat simulation, ferry exhibition with sketches and plates and several models. DSB Museum Trains manage the operation of preserved rolling stock, including short runs to Fruens Bøge and also to Nyborg.